Fig. 4

Fstl1 deletion impairs the morphology of radial glial processes. a to i’ Nestin immunostaining at E12.5 (a and a’), E14.5 (b to c’), E15.5 (d to f’) and E16.5 (g to i’). At E12.5, the development of radial glial fibres was comparable between the WT (a) and Fstl1 ^−/− (a’) cortices. b to c’ At E14.5, many long, parallelly distributed processes spanning the entire cerebral cortex were observed in the WT brains (b and c). In the Fstl1 ^−/− brains, many radial processes were not parallel to one another (b’ and c’). d to i’ At E15.5 and E16.5, the same phenomenon was detected in the RGCs. Scale bars: 100 μm