Fig. 1
From: Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21WAF1/CIP1 expression
LRRK2 phosphorylates p53 at T304 and T377 in an in vitro kinase assay. A. Recombinant GST-ΔN LRRK2 phosphorylates recombinant human p53 WT (A) or mutant proteins B. The recombinant p53 protein was subjected to the in vitro kinase assay with cold ATP (b − d) or γ32P-ATP (a) and analyzed by Western blot with the indicated antibodies (b, p-TXR: phospho-TXR; c, LRRK2: MJFF2; d, p53:DO1) or autoradiography (a). WT: wild type, GS: G2019S, DA: D1994A. C. A schematic diagram of the p53 functional domains and location of the human p53 TXR motifs. Thr 387 which was used as a control was also shown. Numbers below the gel figures are the densitometric results for each band, [radiolabeled p53(a)]/[total p53(d)]