Fig. 5
From: Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21WAF1/CIP1 expression
LRRK2 mediated p53 phosphorylation induces p21 expression and cytotoxicity in differentiated SH-SY5Y cells. The SH-SY5Y cells were transfected with the plasmid expressing shLRRK2 (a), G2019S (b), HA-p53, T304/377D and T304/377A (c), HA- T304/377D (D) or FLAG-p21(E). The p53 T304/377D mutant (d) or p21 (e) were transfected in a dose-dependent manner. The cell lysates were subjected to Western blot with the indicated antibodies with β-actin or α-tubulin as the loading controls. The transfected cells were also subjected to LDH (cytotoxicity) and/or CCK8 (Cell viability) assay (B ~ E). Actual transfection of each plasmid was confirmed by expression of the corresponding proteins. All experiments were repeated three times, and a representative Western image and a graphic representation with the statistical analysis are shown. *: p <0.05; **: p <0.01; ***: p <0.001