Leucine-Rich Repeat Kinase 2 (LRRK2) phosphorylates p53 and induces p21WAF1/CIP1 expression

Table 1 The primers used in the RT-PCR assays

Primer name	Gene	Direction	Sequence	TM (°C)	Product size(bp)
hP21-F	P21	Forward	GTTCCTTGGGAGCCGGAGC	60	417
hP21-R	P21	Reverse	GGTACAAGACAGTGACAGGTC	60	417
LRRK2-7232	LRRK2	Forward	GTCATGATGACAGCACAGC	65	201
LRRK2-7440	LRRK2	Reverse	CTCTTACTCAACAGATGTTCGTCTC	65
pMycXhoI-R	Myc tag	Reverse	CTTCTGAGATGAGTTTTTGTTCCT CGAC	57.3	254^a
b-actin-F	β-actin	Forward	CGCCGCCAGCTCACCATG	62.5	120
b-actin-R	β-actin	Reverse	CACGATGGAGGGGAAGACGG	62.5	120

The polymerase chain reaction was carried out at 95 °C for 1 min and 35 cycles of 95 °C for 30 s, Tm for 30 s, 72 °C for 30 s, and 72 °C for 5 min
^aThe pMycXhoI-R primer was used with LRRK2-7232 to detect expression of Myc-LRRK2 G2019S
Bp base pairs

ISSN: 1756-6606