Fig. 2From: Regulation of STEP61 and tyrosine-phosphorylation of NMDA and AMPA receptors during homeostatic synaptic plasticityProlonged alterations of hippocampal network activity regulate STEP61 activity. a A schematic depicting the regulation of STEP61 activity by its phosphorylation at Ser221 within its kinase-interactive motif, a binding site for all STEP substrates. b–d Immunoblot analysis of STEP61 and Ser221–phosphorylated STEP61 (STEP61-pS221) in hippocampal cultured neurons following CTL, TTX, or BC treatment for 24–48 h (n = 3 per treatment). b, d Prolonged TTX treatment reduced STEP61 protein level and activity. c, d Prolonged BC treatment enhanced STEP61 protein level and activity. d The relative phosphorylation state of STEP61 as calculated by the ratio of STEP61-pS221 level over total STEP61 level. Data shown represent the mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.005)Back to article page