Fig. 4

TAT-STEP WT or C/S blocks the activity-induced bidirectional changes in GluN2B and GluA2. a A simplified schematic (not to scale) of cell-permeable TAT-STEP molecular tools. b–e Immunoblot analysis of hippocampal cultured neurons that were treated for CTL, TTX (b–c), or BC (d–e) for 24 h and 48 h (n = 8 per treatment). Prior to neuronal lysate preparation, neurons were preincubated for 30 min with TAT-myc, TAT-STEP WT, or TAT-STEP C/S proteins. b–c TAT-STEP WT blocked the TTX-induced increase in Tyr¹⁴⁷²–phosphorylation of GluN2B (GluN2B-pY¹⁴⁷²) (b) and 3Tyr-phosphorylation of GluA2 (GluA2-p3Y) (c). d–e TAT-STEP C/S blocked the BC-induced reduction in Tyr¹⁴⁷²–phosphorylation and level of GluN2B (d) as well as GluA2 level but not 3Tyr–phosphorylation of GluA2 (e). Data shown represent the mean ± SEM following normalization to CTL values in the presence of TAT-myc (black bars), TAT-STEP WT (striped bars), and TAT-STEP C/S (gray bars) (*p < 0.05; **p < 0.01; ***p < 0.005)