Fig. 6

Effect of BQ788 and Y-27632 on dysfunction of mitochondrial fission at 3 days after SE. a Western blot images of DRP1, DRP1 S616 and DRP1 S637 in the hippocampus. As compared to vehicle, both BQ788 and Y-27632 (a ROCK inhibitor) attenuate the reductions in DRP1 and DRP1 S616 expression, but increase DRP1 S637 expression. b Quantitative values (mean ± S.E.M) of DRP1, DRP1 S616, DRP1 S637 level (n = 10 per each group). Significant differences from vehicle, *p < 0.05. c Quantitative values (mean ± S.E.M) of DRP1 S616/S637 ratio in the hippocampus (n = 10 per each group). Significant differences from vehicle, *p < 0.05. d Quantitative values (mean ± S.E.M) of mitochondrial length in the CA1 neurons (n = 10 per each group). Significant differences from non-SE animals, *p < 0.05 e-h Representative photographs of mitochondria and NeuN in the CA1 neurons. SE increases mitochondrial length and sphere formation. Both BQ788 and Y-27632 alleviate mitochondrial elongation and sphere formation induced by SE. Bar = 6.25 μm