Table 1 Primary antibodies used in the present study
From: Endothelin-1 induces LIMK2-mediated programmed necrotic neuronal death independent of NOS activity
Antibody | Host | Manufacturer (catalog number) | Dilution used |
|---|---|---|---|
DRP1 | Rabbit | Thermo (PA1-16987) | 1:1000 (WB) |
DRP1 S616 | Rabbit | Cell signalling (#4867) | 1:1000 (WB) |
DRP1 S637 | Rabbit | Cell signalling (#4494) | 1:1000 (WB) |
Endothelin 1 (ET-1) | Rabbit | Abbiotec (250633) | 1:200 (IF) |
Endothelin B receptor (ETB receptor) | Rabbit | Millipore (AB3284) | 1:50 (IF) |
1:1000 (WB) | |||
Glial fibrillary acidic protein (GFAP) | Mouse | Chemicon (MAB3402) | 1:4000 (IF) |
LIMK2 | Rabbit | Abcam (ab45165) | 1:100 (IF) |
1:2000 (WB) | |||
Mitochondrial marker | Mouse | Millipore (MAB3494) | 1:50 (IF) |
NeuN | Mouse | Millipore (MAB377) | 1:500 (IF) |
ROCK1 | Rabbit | Abcam (ab45171) | 1:100 (IF) |
1:1000 (WB) | |||
SMI-71 | Mouse | Covance (SMI-71R) | 1:1000 (IF) |