Fig. 2

Regulation of TGM2 and VEGF. a In situ hybridization (left and middle panel) and emulsion autoradiography (right panel) photomicrographs of TGM2 induction are shown in hippocampal section. The hippocampus is shown at higher magnification in the lower panels. Striking induction in TGM2 is seen in multiple brain regions, including the choroid plexus (CP) after indomethacin administration. Emulsion autoradiographs are representative images from indomethacin treated brains. The vascular expression pattern is indicated by the colored arrows. b TGM2 induction in isolated cortical blood vessels was measured by QPCR. Bar graphs represent the mean from 3 animals, error bars are SEM (p < 0.05). c VEGF expression in vehicle and indomethacin treated animals is shown in the top panel. Cresyl violet stained emulsion autoradiographs of vehicle and indomethacin treated animals is shown in the middle panel. The boxed region in the indomethacin treated section is shown at two levels of magnification in the bottom panel. d VEGF induction in the hippocampus was examined by QPCR. Bar graphs represent the mean from 3 animals, error bars are SEM (p < 0.05). e ELISA analysis of VEGF protein levels in the hippocampus and plasma. VEGF in indomethacin treated animals are shown as a percentage of vehicle VEGF levels. Bar graphs represent the mean from 4 animals, error bars are SEM (p < 0.05)