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Fig. 2 | Molecular Brain

Fig. 2

From: VPS35-deficiency results in an impaired AMPA receptor trafficking and decreased dendritic spine maturation

Fig. 2

Reduced mature spines in VPS35+/m neurons. a Reduced spine density and altered spine morphology in Synapsin-GFP-Actin transfected primary hippocampal neurons from VPS35+/m mice compared with VPS35+/+ controls. Primary hippocampal neurons transfected with Synapsin-GFP-Actin at DIV7 were fixed at DIV15. Top, representative images. Bottom, amplified images. Scale bars, 5 μm. b Quantification of spine density and spine morphology in A. Data were shown as mean ± SEM; n = 30 neurons from 3 independent experiments; *p < 0.05. c Reduced spine density in primary VPS35+/m hippocampal neurons viewed by phalloidin staining analysis. Primary hippocampal neurons were fixed at DIV18 and stained with phalloidin. Scale bars, 5 μm. d Quantification of spine density in C. Data were presented as mean ± SEM; n = 30 neurons from 3 independent experiments; *p < 0.05

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