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Fig. 3 | Molecular Brain

Fig. 3

From: VPS35-deficiency results in an impaired AMPA receptor trafficking and decreased dendritic spine maturation

Fig. 3

Impaired glutamatergic transmission, reduced excitatory synapses, and increased vesicles per synapse in hippocampus of VPS35+/m mice. a Representative traces of mEPSCs in CA1 pyramidal neurons from VPS35+/+ and VPS35+/m mice. Scale bar, 2 s, 10 pA. b Histograms of mEPSC amplitude. n = 15 neurons from 3 mice for each genotype. Student’s t test, *p < 0.05. c Histograms of mEPSC frequency. n = 15 neurons from 3 mice for each genotype. Student’s t test, *p < 0.05. d Decreased number of excitatory synapses and increased number of vesicles per synapse in 4-months old VPS35+/m hippocampus. Top, representative transmission electron microscopic (TEM) images. Red arrows indicate excitatory synapse. Green arrows indicate inhibitory synapse. Scale bar, 200 nm. Bottom, Close up view of one excitatory synapses. e-h Quantification analyses of number of synapses (e), PSD length (f), number of vesicles per synapse(G), and vesicle density in 200 nm*200 nm square shown in yellow color near active zone in D (h). Data were shown as mean ± SEM; n = 30 images from 3 mice per genotype; *p < 0.05

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