Skip to main content
Fig. 2 | Molecular Brain

Fig. 2

From: Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus

Fig. 2

Effect of cAMP activation and glucocorticoid in organotypic culture of rat hypothalamus. a Rat hypothalamic explants were cultured on Millicell membranes. An observation under bright field microscope showed the area of PVN and SCN are intact. b Immunofluorescent staining of AVP-NPII was performed at day 14 to demonstrate survival of vasopressinergic neurons in the culture. The high magnification image of the area in the box is shown. c The mRNA expression of hypothalamic explants in response to treatment of 10 μM FSK and/or 100nM DEX for 4 h (n = 5-7) and 24 h (n = 8-15) was examined by qPCR. Scale bar, 100 μm; Error bar + SEM; *, p < 0.05; **, p < 0.01; ***, p < 0.001 (One way ANOVA); FSK, forskolin; DEX, dexamethasone; PVN, paraventricular nucleus; SCN, suprachiasmatic nucleus. hnAvp, arginine vasopressin heteronuclear RNA

Back to article page