Fig. 1

Aβ oligomers specifically enhance the BACE1 protein level in primary neurons. a Aβ42 oligomers (O) or fibril (F) preparations (2.3 or 4.5 μg) were separated with the Tris/Tricine gel, followed by Western blot analysis with anti-Aβ antibodies, 82E1 or 6E10. b Neurons grown on a 12-well plate were treated with 2.5 μM Aβ as above followed by cell survival assay using the Cell Counting Kit-8, as described in Methods. Relative levels of cell survival are presented as a graph. Data represent means ± SEM from four separate experiments. c Primary cortical neurons (9DIV) grown on a six-well plate were treated with 2.5 μM Aβ oligomers (O), fibrils (F) or vehicle (C) for 2 or 3 days, followed by Western blot analysis with antibodies against BACE1, APP, ADAM10 or β-actin. d Quantitative analysis of BACE1, APP and ADAM10 levels after normalization to β-actin. Data represent means ± SEM from three or four separate experiments. *p < 0.05, **p < 0.01, compared with control