Fig. 2

Aβ oligomers induce activation of caspase 3 and eIF2α, but do not alter GRP78 levels. a Primary neurons were treated with 2.5 μM Aβ oligomers (O), fibrils (F) or vehicle (C) for 2 or 3 days, followed by Western blot analysis of cell lysates. Membranes were probed with the indicated antibodies. b Quantitative analysis of p-eIF2α/total eIF2α ratios and cleaved caspase 3 levels. Data represent means ± SEM from three separate experiments. *p < 0.05 and **p < 0.01, compared with control. c Primary neurons were treated with 2.5 μM Aβ oligomers (O) for 3 days or 1 μM thapsigargin (Thap) for 1 day, followed by Western blot as above. Membranes were probed with the indicated antibodies. d Quantitation of GRP78 levels. Data represent means ± SEM from three independent experiments. **p < 0.01, compared with control