Fig. 3

Aβ oligomers augment BACE1 levels, not at the transcriptional or translational, but the post-translational level. a Total RNA was extracted from neurons treated with 2.5 μM Aβ oligomers (O), fibrils (F) or vehicle (C) for 1 or 2 days, followed by semi-quantitative RT-PCR. b Band intensities of BACE1 and vimentin in (A) were quantified, and BACE1 mRNA levels normalized to those of vimentin presented as a graph. Data represent means ± SEM from three separate experiments. c Primary cortical neurons (8DIV) were infected with recombinant BACE1 adenoviruses expressing rhodopsin-tagged BACE1. The next day, cells were exposed to Aβ oligomers for 1–3 days, followed by Western blot with anti-rhodopsin tag 1D4. Membranes were reprobed with the different antibodies specified. d Quantitative analysis of exogenous BACE1 levels. Data represent means ± SEM from three independent experiments. *p < 0.05, compared with control