Fig. 2

Regulation of Shank3-interacting actin-related proteins by miR-7, miR-34a, and miR-504. a Summary of the process identifying putative miR-7, miR-34a, and miR-504 targets in the Shank3 interactome (top), and the GO analysis of the putative targets (bottom). b Actin-related subnetwork of the Shank3 interactome with color codes for the putative miRNA targets. c miR-7, miR-34a, and miR-504 decreased the expression of the 3′UTRs of some Shank3-interacting proteins in luciferase assays. d Mutation of the miR-7 binding site blocked its repressive effect on the expression of the human PFN2 3′UTR. e Mutation of the miR-504 binding site blocked its repressive effect on the expression of the human SPTBN2 3′UTR. f The sequence alignments across species for the miR-7 and miR-34a binding sites in the PFN2 and SPTBN2 3′UTRs, respectively. All data are presented as mean ± SEM. Statistical analyses are in Additional file 1: Table S3