Fig. 3

Activation of CaMKII-positive neurons in the ACC has no effect on the mechanical threshold in CFA-treated mice. a Schematic of the behavioral experiment. The pre-test was performed three weeks after the virus infusion and optic cannula implantation. After the pre-test, CFA or saline was injected into the sole of the right hind paw. The post-test was done three days after the CFA injection. b Effects of blue light in a ChR2 mouse (left) and pooled data (right) before (black) and after (red) CFA treatment. There was a significant effect of 473 nm laser before CFA (off: 5.00 ± 0.23 g, on: 4.13 ± 0.40 g; n = 8). CFA injection resulted in a lowering of the mechanical threshold and blue light had no effect (off: 3.25 ± 0.25 g, on: 3.29 ± 0.28 g; n = 8) [p < 0.001 in CFA, p = 0.108 in laser, p = 0.120 in CFA and light interaction, two-way repeated measures ANOVA]. c Equivalent results for the saline treated group. There was a significant effect in both the pre-test (off: 4.83 ± 0.15 g, on: 4.12 ± 0.25 g; n = 10) and post-test (off: 4.54 ± 0.11 g, on: 4.08 ± 0.23 g; n = 10) [p = 0.128 in saline, p = 0.027 in laser, p = 0.445 in saline and light interaction]