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Fig. 1 | Molecular Brain

Fig. 1

From: The novel protein kinase C epsilon isoform modulates acetylcholine release in the rat neuromuscular junction

Fig. 1

nPKCε in NMJ. a Western blot analysis of nPKCε and pnPKCε in the synaptic membrane of diaphragm muscles in the control and after incubation with the inhibitor peptide εV1-2 (100 μM). Western blot is shown at the left and quantitation at the right. b Immunohistochemistry in semithin sections from a whole-mount multiple-immunofluorescent stained LAL muscle. nPKCε isoform immunolabaled in green, AChRs in red, and the Schwann cell (S-100, in B1) or syntaxin (Synt, in B2) in blue. c Quantal content, MEPP frequency and MEPP amplitude in basal conditions and after 1 h of incubation with εV1-2 at concentrations of 1–100 μM. d Intracellular recordings show evoked EPPs and spontaneous MEPPs in basal conditions and after incubation with εV1-2. Examples of EPPs (left, horizontal bar 4 ms, vertical bar 5mv) and MEPPs (right, horizontal bar 20 ms, vertical bar 0.5mv) superimposed raw data. e Intracellular recordings show evoked EPPs in basal conditions and after incubation with CaC (horizontal bar 4 ms, vertical bar 5mv). f Raw data shows the effects of PMA in basal conditions and after incubation with εV1-2 on ACh release (horizontal bars 4 ms; vertical bars, left 10 mV, right 5 mV)

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