Fig. 4

nPKCε and the mAChR signaling pathway in diaphragm muscles. Histogram shows changes in ACh release in the presence of mAChR blockers (PIR, 10 μM; MET, 1 μM; AT, 2 μM). We evaluated its effects in basal conditions and after a second incubation with CaC (2: CaC) or εV1-2 (2: εV1-2). We also preincubated with εV1-2 (1: εV1-2) and then blocked mAChR. * p < 0.05 vs. the corresponding control