Fig. 2
From: Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells
Long-term culture of hESC-derived motor neurons. a Immunocytochemical analysis of HB9, ISL-1, βIII-Tubulin, and ChAT after 1, 2, or 4 weeks of monolayer differentiation. Scale bar, 100 μm, and 20 μm (inset). b Quantification of the number of the cells positive for each marker. n = 4, mean ± SEM. *, p < 0.05 (Student’s t test). c Time-course analysis of the expression of PAX6, SOX1, NGN2, OLIG2, NKX2.2, HB9, ISL-1, and ChAT during monolayer differentiation via quantitative RT-PCR. n = 4, mean ± SEM. d Western blot analysis of the expression of the HB9, ISL-1, and ChAT proteins during monolayer differentiation. e Quantitative analysis of the expression of the HB9, ISL-1, and ChAT proteins through densitometry using ImageJ. The protein expression levels are normalized to β-Actin. n = 4, mean ± SEM. *, p < 0.05, (Student’s t test)