Fig. 3
From: Rapid, efficient, and simple motor neuron differentiation from human pluripotent stem cells
Co-culture of hESC-derived motor neurons with myotubes. a Motor neurons derived from KhES1 cells were plated on myotubes derived from the human myoblast cell line Hu5/E18 to form end plate-like structures. Scale bar, 20 μm. b Immunocytochemical analysis of βIII-Tubulin and MyHC after 3 days of co-culture with motor neurons and myotubes. AChR clusters were visualized with Alexa 555-conjugated α-Bungarotoxin (α-BTX, white allows). Scale bar, 50 μm