Table 1 Primer sequences and cycling conditions for quantitative RT-PCR analysis

SOX1

hSOC1-S3755

GAAATAGCCAATGCCAGGTG

hSOX1-AS4039

CCGTGAATACGATGAGTGTTACC

285

62

-

PAX6

hPAX6-S421

TAGGGGCGCGCAGATGTGTG

hPAX6-AS538

TGCATGCTGGCTCTGGCTGG

118

68

-

OLIG2

hOLIG2-S49

GTTCTCCCCTGAGGCTTTTC

hOLIG2-AS223

AGAAAAAGGTCATCGGGCTC

175

67

-

NKX2.2

hNKX2.2-S434

GCTCTGTGGCCGAAGGTCCG

hNKX2.2-AS657

GCTTGAGTCCTGAGGGGGCG

224

62

-

ISL-1

hISL1-S1294

AGCAGCCCAATGACAAAACT

hISL1-AS1494

CTGAAAAATTGACCAGTTGCTG

201

60

-

HB9

hHB9-S951

GTCCACCGCGGGCATGATCC

hHB9-AS1162

TCTTCACCTGGGTCTCGGTGAGC

212

62

-

CHAT

hChAT-S1360

GGAGGCGTGGAGCTCAGCGACACC

hChAT-AS1592

CGGGGAGCTCGCTGACGGAGTCTG

256

62

-

NGN2

hNGN2-S93

AGACACGCACCACCACCACAACAC

hNGN2-AS293

TGACTTTGGCCTGTGCCGGGAATC

201

60

+

EBNA-1 tg

EBNA-1-S

ATCAGGGCCAAGACATAGAGATG

EBNA-1-AS

GCCAATGCAACTTGGACGTT

61

60

-

NANOG total

hNANOG-S286

CCTATGCCTGTGATTTGTGG

hNANOG-AS503

TGTTTCTTGACTGGGACCTTG

218

62

-

OCT4 total

hPOU5F1-S1165

GACAGGGGGAGGGGAGGAGCTAGG

hPOU5F1-AS1283

CTTCCCTCCAACCAGTTGCCCCAAAC

144

62

-

OCT4 tg

hPOU5F1-pla-S

CATTCAAACTGAGGTAAGGG

hPOU5F1-pla-AS

TAGCGTAAAAGGAGCAACATAG

124

62

-

SOX2 tg

hSOX2-pla-S

TTCACATGTCCCAGCACTACCAGA

hSOX2-pla-AS

TTTGTTTGACAGGAGCGACAAT

111

62

-

KLF4 tg

hKLF4-pla-S

CCACCTCGCCTTACACATGAAGA

hKLF4-pla-AS

TAGCGTAAAAGGAGCAACATAG

156

62

-

L-MYC tg

hL-MYC-pla-S

GGCTGAGAAGAGGATGGCTAC

hL-MYC-pla-AS

TTTGTTTGACAGGAGCGACAAT

122

62

-

LIN28 tg

hLIN28-pla-S

AGCCATATGGTAGCCTCATGTCCGC

hLIN28-pla-AS

TAGCGTAAAAGGAGCAACATAG

251

62

-

β-ACTIN (human specific)

hb-ACTIN-S1062

GATCAAGATCATTGCTCCTCCT

hb-ACTIN-AS1241

GGGTGTAACGCAACTAAGTCA

180

62

-