Fig. 1
Protein list upregulated by Aβ1-42 and their quantification results from a cytokine-array analysis (a). The list of reproducibly upregulated proteins was obtained from three independent cytokine-array analyses. Proteins related to inflammation and cell adhesion were shown to be upregulated. Real data and quantitative analyses are shown (b, c). The culture medium from primary murine astrocytes was collected after a 48-h treatment with 1 μM Aβ1–42 or synthetic peptide Aβ42-1 (inverted sequence of Aβ) and was subjected to analysis. The left spots are the results obtained for Aβ1–42, and the right spots are the results obtained for the control (b). On the membrane of the cytokine-array analysis, Anti-HRP and anti-streptavidin are laid out as positive control signals, which can be used to identify the orientation and help normalize the results from the different arrays being compared. As a result of densitometry for these normalized spots, proteins that had significantly increased were selected