Fig. 2

Characteristics of general health, sensory and motor function in Hbegf cKO mice. No significant changes in body weight: a, body temperature: b, neuromuscular strength in the wire hang test: c, thermal-nociceptive threshold using the thermal stimulator: d, or the electrical threshold of peripheral nerve (Aβ, Aδ, C-fiber) using a Neurometer CPT system: e were observed. No significant change in the latency to fall in the rotarod training: f, and test period using different speeds of 10, 20, 30, and 40 rpm: g, h, No significant change in the stationary horizontal thin-rod test. No significant change in the accelerating rotarod test in repetitive trials: i, and with differential speeds: j. During the training period, each mouse was placed on the rotarod revolving at a constant speed (4.5 rpm) and then accelerated to 45 rpm. Mice were trained for 3 consecutive days, receiving four trials per day with a 1 h inter-trial interval: (i). During the test period on day 4, mice were tested in seven consecutive 60 s trials at constant speeds of 5, 12, 18, 25, 31, 40 and 45 rpm. The mean latency to fall from the rotarod (for the three trials at each speed level) was recorded: (j). n = 7 (Control), n = 7 (cKO). All results are presented as means ± s.e.m