Fig. 4

Hbegf deficiency aters neurogenesis in Hbegf cKO mice. a Representative pictures of BrdU-positive neurons. The specimens show BrdU-positive neurons in the subgranular zone (SGZ), subventricular zone (SVZ), rostromedial striatum (RMS) and olfactory bulb (OLF). Scale bar: 100 μm. b Comparison of the numbers of BrdU-positive neurons. c Representative pictures of detailed localization of doublecortin (DCX)-positive immature neurons in the dentate gyrus. d Sub-localization analysis of DCX-positive immature neurons. #1 (between of hilus and SGZ), #2 (SGZ and inner 1/3 of region of granular cell layer/GCL), #3 (medial 1/3 of GCL), #4 (outer 1/3 of GCL), #5 (molecular layer/MOL). e, f Quantitative analysis of the number of DCX-positive cells and their relative positions in dentate gyrus. e Total number of DCX positive cells in DG. f The number of DCX positive cells in each layer (#1 - #5). n = 4 (Control), n = 4 (cKO). *p <0.05 (control versus cKO). Values are the means ± s.e.m