Fig. 1

Expression of Huwe1 and Gadd45b in cortical neuronal cells after OGD/R. Primary cortical neurons were subjected to 3 h OGD followed by reperfusion for 6 h, 24 h, 48 h, 72 h and 120 h. a Oxygen glucose deprivation and reperfusion decreased the cell viability, compared to normal neuron cells control. Cell viability was analyzed by CCK-8 assay. b Oxygen glucose deprivation and reperfusion induced cortex neuron cells death. Cell death was evaluated by LDH assay. c Western blot assay of Huwe1 and Gadd45b expression at the indicated time points after OGD/R. d The quantitative results from (c). Tubulin was used as an internal control. *p < 0.05 versus control. ^@ p < 0.05 versus OGD/R 24 h