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Fig. 6 | Molecular Brain

Fig. 6

From: Huwe1 interacts with Gadd45b under oxygen-glucose deprivation and reperfusion injury in primary Rat cortical neuronal cells

Fig. 6

The immunofluorescent results of Gadd45b and BDNF. Neuron cells on coverslips were infected with lentivirus shRNA-Huwe1 or shRNA-Gadd45b, followed by OGD and reperfusion for 24 h. Neuron cells were incubated with the anti-Gadd45b antibody (Red) and anti-BDNF antibody (Green) for double-labeled immunofluorescence assay. The immunofluorescence of Gadd45b and BDNF is mainly around the cell nucleus in normal cortex neuron cells (a). Treatment with shRNA-Gadd45b (c) decreased the expression of Gadd45b and BDNF, compared to the lentivirus-scramble control group (b) at 24 h. Treatment with shRNA-Huwe1 (e) increased the expression of Gadd45b and BDNF, compared to the lentivirus-GFP-scramble control group (d) at 24 h. DAPI (blue) is used for cell nucleus staining. Scale bar = 10 μm

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