Fig. 7
The expression of BDNF receptors and downstream at 24 h after reperfusion. Neuron cells were infected with lentivirus shRNA-Huwe1 or lentivirus shRNA-Gadd45b, followed by OGD and reperfusion 24 h. Cells were collected for western blot. a Inhibition of Gadd45b decreased the protein levels of TrkB, p-Erk1/2 and p-CREB and increased the expression of p75NTR, compared to the lentivirus-scramble control group (LV-ctrl). ShRNA-Huwe1 increased the expression of TrkB, p-Erk1/2 and p-CREB, and decreased the expression of p75NTR, compared to lentivirus-GFP-scramble control group (LV-GFP-ctrl). b Neuron cells were prepared for checking the activity of RhoA at different groups, by measuring absorbance at 490 nm using a microplate spectrophotometer. Inhibition of Gadd45b decreased the activity of RhoA. c The proposed scheme model for the effect of Huwe1 on Gadd45b and BDNF signal in a ubiquitination-dependent manner under oxygen-glucose deprivation and reperfusion injury in primary rat cortical neuronal cells. Tubulin was used as an internal control for western blot; # p < 0.05 versus LV-GFP-ctrl or LV-ctrl