Fig. 2
The MAFE assay detects the effects of food deprivation and gut stretch on food ingestion. a Volume of 100 mM sucrose consumed in a meal, by fed Canton-S flies, and those starved for 12, 24 and 36 h (n = 25-29). b Duration (x-axis) and volume of 100 mM sucrose consumed (y-axis) in the first feeding bout by Canton-S flies starved for 12 and 36 h (n = 29-34). The regressed linear equation and the R2 value are listed in the plot. c Feeding speed of Canton-S flies starved for 12 and 36 h (n = 29-34). d Volume of 100 mM D-glucose consumed in a meal, by fed flies with indicated genotypes at 20 °C or 30 °C (n = 24-39). e Volume of 100 mM D-glucose consumed in a meal, by starved (24–36 h) flies with indicated genotypes at 20 °C or 30 °C (n = 19-35). Sample size for each data set was summarized in Tab. S1. Error bars represent SEM. ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. One-way ANOVA followed by Bonferroni post hoc test was used for comparisons for more than 2 groups. Two-way ANOVA (and post hoc test if applicable) was applied for comparisons with more than one variant