Fig. 3

Starved flies can assess food nutritional value and modulate food ingestion. a, c Fractions of Canton-S flies exhibiting PER responses to (a) 100 mM D-/L-glucose and (c) 100 mM D-/L-fructose when starved for 12, 24, and 36 h (n = 19-36). b, d Volume of (b) 100 mM D-/L-glucose and (d) 100 mM D-/L-fructose consumed in a meal, by Canton-S flies starved for 12, 24 and 36 h (n = 19-36). e Volume of sugar solutions with indicated D- and L-glucose concentrations consumed in a meal, by 36-h starved Canton-S flies (n = 22-35). f Fractions of 36-h starved Canton-S flies exhibiting PER responses to the indicated second sugar (“Re-stimulated with”) after finishing a meal with the indicated first sugar (“Fed with”) (n = 25-26). g Feeding time of 36-h starved Canton-S flies when fed with 100 mM D-/L-glucose (n = 24-30). h Volume of 100 mM D-/L-glucose consumed during the first feeding bout of a meal by 36-h starved Canton-S (n = 68-69). Sample size for each data set was summarized in Tab. S1. Error bars represent SEM. ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Student’s t-test was used for pair wise comparisons. One-way ANOVA followed by Bonferroni post hoc test was used for comparisons for more than 2 groups. Two-way ANOVA (and post hoc test if applicable) was applied for comparisons with more than one variant