Fig. 4
From: Rasd1, a small G protein with a big role in the hypothalamic response to neuronal activation
DEX induced Rasd1 mRNA expression in the rat PVN and SON. a the addition of DEX to hypothalamic organotypic culture media 4 or 24 h before collection of samples increased the expression of Rasd1 in the PVN and SON compared to vehicle (DMSO) controls. b rats were injected with DEX (1 mg/kg) or vehicle (0.15 M NaCl) 2 h before i.p injection of IS (0.15 M NaCl) or HS (1.5 M NaCl; 1.5 ml/100 g body weight). Brains were collected 30 min after IS or HS injection. qPCR was performed to analyse the expression of Rasd1 c-Fos, Nr4a1, hnAvp and hnCrh in PVN and SON. c to block endogenous glucocorticoid synthesis rats were injected with MET or a combination of MET and DEX 4 h before i.p injection of IS or HS. Brains were collected after 30 min and qPCR analysis was performed on the cDNAs generated. a, independent-sample unpaired Student’s t tests; b–c, Two-way ANOVA. Values are means + SEM of n = 5−8 animals per group. *p < 0.05, **p < 0.01, ***p < 0.001. DEX, dexamethasone; MET, Metyrapone; VEH, vehicle