Fig. 5

Rasd1 mediates actions of glucocorticoid on transcription factors c-Fos and Nr4a1 in AtT20 cells. a, Rasd1 mRNA expression in response to 1 h treatment with different concentrations of DEX. b changes in Rasd1 mRNA expression after treatment with 100nM DEX over a 4 h experimental period compared to time matched vehicle controls. c responses to Rasd1 knock down cell lines to 1 h treatment with 100nM DEX were examined by qPCR compared to a control non-targeting shRNA cell line. d the effect of pretreatment with DEX (2 h), 72 h of adenoviral mediated Rasd1 overexpression and Rasd1 knockdown on FSK (10 μM) induced c-Fos and Nr4a1 mRNA expression was examined compared to controls. e cells were infected with Rasd1 adenovirus for 4 h. Media was replaced and cells were grown in the presence of farensyltransferase inhibitor FTI-277 for 72 h and qPCR analysis of c-Fos and Nr4a1 expression was performed. f the effect of 72 h of adenoviral mediated dnRasd1 overexpression on FSK induced c-Fos and Nr4a1 mRNA expression was examined compared to controls. g responses of GR knockdown cell lines to treatment with 100nM DEX were examined by qPCR compared to a control non-targeting shRNA cell line. VEH, vehicle; NT, non-targeting; Con, control. Values are means + SEM of n = 3−4 per group. *p < 0.05, **p < 0.01, ***p < 0.001