Fig. 4

Ectopic expression of TH in the cerebellum of Lrp5/6 dCKO mice. (a-c) A few TH-positive cells are scattered in the lateral portion of the cerebellum of P13 Lrp5/6 dCKO mice, and they are Calbindin-positive. (d-f) The distribution of TH expression (triangles), shown by immunostaining (d, e) and in situ hybridization (f), is expanded, and TH-expressing cells are present throughout the cerebellum of Lrp5/6 dCKO mice at P17 (d, e) and P19 (f). Note that endogenous TH expression is present in the locus coeruleus (LC) (d, f). (e) Higher magnification of the boxed area in (d). (g, h) No TH transcripts or immunoreactivities are detected in WT cerebellum. (i) Western blot showing TH expression in Lrp5/6 dCKO mice but not in wild-type or Lrp5 CKO or Lrp6 CKO mice. Molecular weight is indicated. Scale bars, 30 μm in (c) and applies to (a, b), 270 μm in (d), 295 μm in (f), 100 μm in (g, h), and 40 μm in (e)