Fig. 3
From: ASIC subunit ratio and differential surface trafficking in the brain
Developmental changes in ASIC expression in the brain. a Representative Western blots showing ASIC1a, 2a, 2b expression at different postnatal stages. Blots shown are representative from 4 separate repeats. b Quantification showing the relative ASIC1a expression level at different age. Numbers on the bars are number of animals analyzed. c Quantification of relative ASIC2a and 2b protein level in WT and ASIC1a-/- brain. Asterisks indicate significant differences (p = 0.009 for ASIC2a, p < 0.001 for ASIC2b, Student's t-test). d Quantification of relative expression of ASIC1a:2a:2b in the brain at different postnatal ages. Since we normalized the ratio using the 1a-2a construct, the numbers obtained represent the ratio of the number of subunits