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Fig. 5 | Molecular Brain

Fig. 5

From: ASIC subunit ratio and differential surface trafficking in the brain

Fig. 5

Differential surface trafficking of ASIC1a, 2a, and 2b in the brain. a Western blot showing surface and total ASIC levels in acutely dissected brain tissue. Different brain regions (as indicated) were dissected from 3 to 5 week old mice. Surface fractions were isolated by surface biotinylation and NeutrAvidin pull down as described in Methods. Membranes were blotted for ASIC1a and ASIC2. b & c Quantification showing ASIC1a:2a (b) and 1a:2b (c) ratio in the corresponding brain regions. We quantified 1a:2b ratio for cerebellum because there was little ASIC2a in cerebellum

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