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Fig. 1 | Molecular Brain

Fig. 1

From: PINK1 expression increases during brain development and stem cell differentiation, and affects the development of GFAP-positive astrocytes

Fig. 1

PINK1 expression increased during brain development, and PINK1 deficiency caused defects in GFAP expression. a, b Mouse brains were collected at the indicated ages. The levels of a neuron marker (TUJ1), an astrocyte marker (GFAP), an oligodendrocyte marker (MBP), and PINK1 were assayed by Western blotting, with GAPDH used as the loading control (a). The mRNA levels of PINK1 during brain development were determined using Q-PCR (b). c At postnatal day 1, postnatal day 8 and 8 weeks, whole brains from WT and PINK1-KO mice were collected and GFAP, TUJ-1, and MAP2 protein levels were analyzed by Western blotting. The band intensities of GFAP, TUJ1 and MAP2 were quantified and normalized with respect to that of GAPDH. The data shown are representative of two independent experiments (a, b). Values in (b, c) are means ± SEMs of at least four samples (**, P < 0.01)

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Molecular Brain

ISSN: 1756-6606