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Fig. 4 | Molecular Brain

Fig. 4

From: PINK1 expression increases during brain development and stem cell differentiation, and affects the development of GFAP-positive astrocytes

Fig. 4

PINK1 deficiency did not affect GFAP mRNA expression or the signaling pathways involved in astrocyte differentiation. a, b The levels of proteins known to be involved in astrocyte differentiation-related pathways (e.g., p-SMAD1/5/8, p-STAT3, and HES1) were measured by Western blotting (a). GAPDH was used as the loading control. The band intensities of p-SMAD1/5/8, p-STAT3, and HES1 were quantified in (b). c GFAP mRNA levels were measured in WT and PINK1-KO NSCs using Q-PCR on days 0, 1, 3, and 5 of differentiation. d, e WT and PINK1-KO NSCs were differentiated in the presence of 0.1 ng/ml CNTF for 5 days. p-STAT3 levels were compared after the indicated durations of differentiation (d), and GFAP mRNA levels were examined on day 3 of differentiation (e). Values are means ± SEM of at least four samples (b, c, e)

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Molecular Brain

ISSN: 1756-6606