Fig. 5

PINK1-KO NSCs exhibited normal mitochondrial functions during differentiation. a On day 5 (left panel) or the indicate times of differentiation (right panel), intracellular ROS levels and the mitochondrial membrane potential were monitored by loading cells for 30 min with 10 μM carboxyl-H2DFFDA and 125 nM MitoTracker Red CMXRos, respectively. b On days 1, 3, and 5 of differentiation, the content of mitochondrial DNA relative to that of nuclear DNA was measured as the ratio of the mitochondrial D-loop (mito-D-loop) to the nuclear-encoded GAPDH gene, using Q-PCR as described in Methods. Data are means ± SEM of three samples. The data shown are representative of at least three independent experiments