Fig. 7

Altered distribution of PSD-95 in netrin-G2 KO mice. a and b Western blot analyses for postsynaptic molecules in the cerebral samples. There were no detectable changes in the amounts of molecules examined (sample = animal numbers are indicated in the columns). c Immunohistochemical staining for PSD-95 in the dentate gyrus of WT, netrin-G1 KO, and netrin-G2 KO mice. White arrowheads indicate approximate borders among OML, MML, and IML. The difference in fluorescence intensity between MML and IML was much more distinct in netrin-G2 KO mice compared to WT and netrin-G1 KO mice (scale bar: 150 μm). d and e Relative intensities of PSD-95 in each layer were first calibrated by MAP-2 intensity, and then the ratio of the V_OML/V_IML, V_MML/V_IML, and V_SR/V_SL values were calculated for further normalization. The relative intensity of PSD-95 was selectively decreased in the MML of netrin-G2 KO mice (1-way ANOVA, **P < 0.01 post-hoc Scheffe’s test). Intensities were measured from 5 ROI/layer/slice, 6 slices/animal, and 3 mice for each genotype. Total numbers of ROIs are indicated in the columns. IML, inner molecular layer; MML, middle molecular layer; OML, outer molecular layer; SL, stratum lacunosum-moleculare; SR, stratum radiatum. Data are presented as mean ± SEM