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Fig. 4 | Molecular Brain

Fig. 4

From: Reduction in parvalbumin expression not loss of the parvalbumin-expressing GABA interneuron subpopulation in genetic parvalbumin and shank mouse models of autism

Fig. 4

a Left: stereological estimations of PV+ (light gray) and VVA+ (dark gray) cells in SSC of WT and Shank1-/- PND25 male mice. Middle: estimation of double-labeled cells in SSC of PND25 WT and Shank1-/- mice. Right: percentage of PV+ cells surrounded by VVA (light gray) and percentage of VVA+ cells displaying PV expression and thus identified as PV+ cells (dark grey) in WT and Shank1-/- mice. b Representative Western blot and quantification of PV protein levels in Shank1-/- mice. CB signals were used as loading controls and served for the normalization of the PV signals. Data are from three independent experiments and are shown as mean ± SEM. Results are expressed as percentage of normalized PV levels measured in control (WT) samples, defined as 100 %. c Quantitative RT-PCR analysis of forebrain samples of Shank1-/- mice. PV (Pvalb) mRNA levels were normalized to 18S mRNA levels and expressed as fold change. Data from three independent experiments were pooled together and are shown as mean ± SD. In all graphs, asterisks indicate statistical significance vs. WT (p-value <0.05, p = 0.0006)

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Molecular Brain

ISSN: 1756-6606