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Fig. 6 | Molecular Brain

Fig. 6

From: Reduction in parvalbumin expression not loss of the parvalbumin-expressing GABA interneuron subpopulation in genetic parvalbumin and shank mouse models of autism

Fig. 6

a) Left: stereological estimations of PV+ (light gray) and VVA+ (dark gray) cells in the striatum of WT and Shank3B-/- PND25 male mice. Middle: estimation of double-labeled cells in SSC of PND25 WT and Shank3B-/- mice. Right: percentage of PV+ cells surrounded by VVA (light gray) and VVA+ cells with PV expression (dark grey) in WT and Shank3B-/- mice. b) Representative Western blot and quantification of PV protein levels in Shank3B-/- mice. CB was used as loading control for the normalization of the PV signal, since CB expression levels were unchanged in Shank3B-/- mice (data not shown). Data are from three independent experiments and are shown as mean ± SEM. Results are expressed as a percentage of normalized PV levels measured in control (WT), defined as 100 %. c) Quantitative RT-PCR analysis of forebrain samples of Shank3B-/- mice. PV (Pvalb) mRNA levels were normalized to 18S mRNA levels and expressed as fold change. Data from three independent experiments were pooled together and are shown as mean ± SD. In all graphs, asterisks indicate statistical significance vs. WT (p-value <0.05, p = 0.0002)

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Molecular Brain

ISSN: 1756-6606