Table 1 Stereological sampling parameters
Brain region | Cutting plane | No. of sections | Section evaluation interval | Height of disector (μm) | Guard zone height (μm) | Counting frame area (μm) | Sampling grid area (μm) | Measured section thickness mean (μm) | CE |
|---|---|---|---|---|---|---|---|---|---|
mPFC | coronal | 4-5 | 6 | 20 | 0.5 | 90 × 70 | 150 × 200 | 22.4 | CEm=10.07 ≤ CE ≤ CEm=0 0.09 |
Striatum | coronal | 7-8 | 6 | 20 | 0.5 | 110 × 90 | 300 × 300 | 23.0 | CEm=10.06 ≤ CE ≤ CEm=00.09 |
SSC | sagittal | 10-12 | 6 | 20 | 0.5 | 583.5 × 335.6 | 250 × 250 | 23.4 | CEm=10.07 ≤ CE ≤ CEm=00.10 |