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Fig. 1 | Molecular Brain

Fig. 1

From: Endothelial progenitor cells and neural progenitor cells synergistically protect cerebral endothelial cells from Hypoxia/reoxygenation-induced injury via activating the PI3K/Akt pathway

Fig. 1

Characterization of EPCs and NPCs from human iPSCs. A1, iPSCs grew on feeder-free matrigel. Scale bar: 2000 μm. A2-A3, iPSCs expressed Sox2 and Oct3/4. B1, NPCs grew as neurospheres after 7 days neural differentiation. B2-B4, generated NPCs positively expressed neural progenitor markers nestin and Pax6, and were negative for pluripotent stem cell marker Oct3/4. C, generated neurons positively stained neuron specific marker Tuj1. D1-D3, purified EPCs positively expressed endothelial progenitor markers CD34 and KDR, but were negative for Oct3/4. Black curves: isotype control; red curves: antibodies. D4, tube formation of EPCs. DAPI counterstained cell nucleus. Scale bar: 200 μm from A2-D4

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Molecular Brain

ISSN: 1756-6606