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Fig. 2 | Molecular Brain

Fig. 2

From: Endothelial progenitor cells and neural progenitor cells synergistically protect cerebral endothelial cells from Hypoxia/reoxygenation-induced injury via activating the PI3K/Akt pathway

Fig. 2

EPCs and NPCs promoted the survival of H/R-injured ECs via activating the PI3K pathway. MTT assay and PI/FITC-Annexin V apoptosis assay were conducted on H/R-injured ECs co-cultured with EPCs and/or NPCs for 24 h as described in Material and Methods. A1, representative morphology images showing the viability of ECs. A2, summarized data showing EC viability which is synergistically increased when co-cultured with the combination of EPCs and NPCs than that co-cultured with EPCs or NPCs alone. B1, representative flow plots of EC apoptotic rate. B2, summarized data of the apoptotic rate of ECs, showing that the combination of EPCs and NPCs offers better anti-apoptotic effect than EPCs or NPCs alone. Block the PI3K pathway could diminish the beneficial effects of EPCs and/or NPCs. And the PI3K pathway upstream blockers, SU1498 and K252a, reduced these effects of EPCs and NPCs. *p < 0.05, vs. Normoxia; #p < 0.05, vs. vehicle. Data are expressed as mean ± SEM, n = 6/group/measurement. LY294002: PI3K inhibitor; SU1498: VEGFR2 inhibitor; K252a: TrkB inhibitor

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