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Fig. 5 | Molecular Brain

Fig. 5

From: RAB18, a protein associated with Warburg Micro syndrome, controls neuronal migration in the developing cerebral cortex

Fig. 5

Knockdown of RAB18 impairs the surface expression level of N-cadherin in neuron. a Representative images of cortical neurons stained for N-cadherin (red), RAB18 (green) and DAPI (blue). Scale bar: 5 μm. b Representative images of surface N-cadherin immunostaining of cortex neurons infected with lentivirus driving the expression of control or shRAB18-3. Scale bar: 5 μm. c Quantification of surface N-cadherin from neurons infected with lentivirus expressing control or shRAB18-3 (n = 10, * p < 0.05, t test). d Immunoblot analysis of membrane proteins of cortical neurons infected with indicated plasmids with the indicated antibodies. e The graph indicates the ratio of N-cadherin / NaK ATPase. Knockdown of RAB18 significantly decreased N-cadherin protein level in the membrane of neurons (n = 5, * p < 0.05, t test). f Representative coronal section showing migration of transfected neurons 3 d after electroporated at E14.5 with GFP plasmid together with indicated constructs (shRAB18-3 + pCAG, n = 4; shRAB18-3 + N-cadherin, n = 4). Scale bar: 100 μm. g Quantification of percentages of GFP positive neurons in different cortical regions. Data represent the mean ± SEM and were analyzed by t test; *** p < 0.001 and ** p < 0.01 versus shRAB18-3 + pCAG

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