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Fig. 4 | Molecular Brain

Fig. 4

From: Wnt3a upregulates brain-derived insulin by increasing NeuroD1 via Wnt/β-catenin signaling in the hypothalamus

Fig. 4

Knockdown of NeuroD1 reduces the expression of Ins2. NeuroD1 was knocked down by infecting N39 cells with lentivirus containing shRNA (shNeuroD1) for 72 h. a Knockdown efficiency was determined by examining the levels of NeuroD1 mRNA using qRT-PCR (n = 23). b The level of NeuroD1 protein was assessed by immunoblot assay, and c the intensity of NeuroD1 bands was quantified using the ImageJ software with normalization to GAPDH (n = 6). d The basal expression of Ins2 was determined by qRT-PCR after infection with shRNA lentivirus against NeuroD1 for 72 h (n = 12). Data are means + SEM. **p < 0.01, ***p < 0.001 compared with non-targeting shRNA lentivirus-infected cells (shNon-target)

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Molecular Brain

ISSN: 1756-6606