Fig. 4

Sevoflurane and desflurane inhibit neurite growth, which is restored with Mdivi-1 pre-treatment. (a–f) Phase contrast images of neurons after exposure to the indicated anesthetics in the presence or absence of Mdivi-1 at 3 days post-culture. (g–l) Confocal microscopy images of areas stained with 160 kDa neurofilament polyclonal antibodies. (m) Quantification of neurofilament staining with automated neurite tracing software, NeuriteTracer. Two-way ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001 as determined by pairwise comparison of the post-hoc tests. Scale bar 25 μm (a–f), 100 μm (g–l)