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Fig. 4 | Molecular Brain

Fig. 4

From: Electrophysiological behavior of neonatal astrocytes in hippocampal stratum radiatum

Fig. 4

Complex expression of rectifying K+ conductances in neonatal astrocytes. a To maximally induce outward IKa, and potential inward INa, a −110 mV/300 ms prepulse was followed by a series of 50 ms steps from −180 to +40 mV with 10 mV increments. Absence of any depolarization-induced inward INa was shown in the inset below (a) after symmetric subtraction of leak and capacitive currents. b To eliminate IKa, a prepulse at −40 mV/300 ms was followed by test steps from −180 to +40 mV with 10 mV increments and 50 ms duration. c, d The isolated IKa and its corresponding I-V plot with a V rev at −40 mV. The IKa was isolated by off-line subtraction of recording (a and b). e, f Isolated IKd and its corresponding I-V plot. The IKd was isolated by symmetric leak and capacitive current subtraction from recording (b). g Inward K+ conductance (IKin) in a P1 astrocyte induced after inhibition of gap junction coupling by incubation of slice with 100 μM MFA. For IKin activation, a 500 ms pre-pulse at 0 mV was followed by a set of steps from −180 to 0 mV, 50 ms duration and 10 mV increments. h Addition of 100 μM Ba2+ for 15 min substantially reduced the initial IKin. i Subtracted Ba2+-sensitive currents. j I-V plots illustrate the total IKin, Ba2+-sensitive IKin and the remaining outward Ba2+-insensitive currents as indicated by color codes. The Ba2+ -sensitive and insensitive conductances were reversed at −79.7 mV and 79.7 mV, respectively

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