Fig. 5
From: Electrophysiological behavior of neonatal astrocytes in hippocampal stratum radiatum
Expression of multiple leak type K+ conductances contributing to the resting VM of neonatal astrocytes. a, b. Whole-cell recording from a P1 wild type and P1 TWIK-1/TREK-1 double gene double knockout (dKO) astrocyte as indicated. Prior to recording, gap junction coupling was inhibited by 100 μM MFA, and Kir4.1 was inhibited by 100 μM Ba2+. c I-V plots of recording (a and b) as indicated by color codes. The V rev was −69.9 and −68.5 mV in recorded WT and dKO astrocytes, respectively. d The V M values recorded from WT and dKO astrocytes under various conditions indicated. A quasi-physiological V M was retained in both WT and dKO astrocytes in the absence of Kir4.1 conductance