Fig. 1

Optimization of protein synthesis inhibition. a–c The effect of anisomycin (Ani) dose on protein synthesis inhibition following electric convulsive stimulation (ECS). d–f, The effect of 600 μg anisomycin injection time on protein synthesis inhibition following ECS. a and d Experimental design. b and e Representative immunohistochemically stained images of c-fos (green) and NeuN (red) expression in the dentate gyri ipsi- and contra-lateral to the i.c.v. injection side. c and f, Quantitation of c-fos signal in (b) and (e), respectively. n = 3–4 rats per condition. (** p < 0.01; **** p < 0.0001; ns, not significant (p > 0.05); two-way ANOVA, Tukey’s posthoc for within group test compared to PBS group without ECS except otherwise indicated and Sidak posthoc for test between ipsi- and contra-lateral sides). No significant difference between ipsi- and contra-lateral sides at all points. Error bars indicate SEM